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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- ELISA [1]
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- Product number
- 102-PA28 - Provider product page
- Provider
- ReliaTech GmbH
- Product name
- PRAME
- Antibody type
- Polyclonal
- Antigen
- Recombinant human PRAME
- Description
- antibody Protein-A purified from serum
- Reactivity
- Human
- Host
- Rabbit
- Antigen sequence
MNPLETLSITNCRLSEGDVMHLSQSPSVSQLSVLS
LSGVMLTDVSPEPLQALLERASATLQDLVFDECGI
TDDQLLALLPSLSHCSQLTTLSFYGNSISI- Antibody clone number
- Rabbit IG
- Vial size
- 200 µl
- Storage
- Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
- Handling
- Restore in sterile water to a concentration of 0.1-1.0 mg/ml. The antibody solution should be gently mixed before use.
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Supportive validation
- Submitted by
- ReliaTech GmbH (provider)
- Main image
- Experimental details
- Western Analysis of anti-human PRAME. Sample was loaded in 21% SDS-polyacrylamide gel under reducing conditions. Lane 1: MWM (kDa); lane 2: rh PRAME.
- Sample type
- Purified recombinant proteins
Supportive validation
- Submitted by
- ReliaTech GmbH (provider)
- Main image
- Experimental details
- ELISA using the polyclonal rabbit anti-human PRAME as detection antibody and the recombinant human PRAME fragment (ER #400-016) as standard.
