Antibody data
- Antibody Data
 - Antigen structure
 - References [0]
 - Comments [0]
 - Validations
 - Western blot [1]
 - Immunocytochemistry [1]
 
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Validation data
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- Product number
 - H00023532-D01P - Provider product page

 - Provider
 - Abnova Corporation
 - Proper citation
 - Abnova Corporation Cat#H00023532-D01P, RRID:AB_1711691
 - Product name
 - PRAME purified MaxPab rabbit polyclonal antibody (D01P)
 - Antibody type
 - Polyclonal
 - Description
 - Rabbit polyclonal antibody raised against a full-length human PRAME protein.
 - Antigen sequence
 MERRRLWGSIQSRYISMSVWTSPRRLVELAGQSLL
KDEALAIAALELLPRELFPPLFMAAFDGRHSQTLK
AMVQAWPFTCLPLGVLMKGQHLHLETFKAVLDGLD
VLLAQEVRPRRWKLQVLDLRKNSHQDFWTVWSGNR
ASLYSFPEPEAAQPMTKKRKVDGLSTEAEQPFIPV
EVLVDLFLKEGACDELFSYLIEKVKRKKNVLRLCC
KKLKIFAMPMQDIKMILKMVQLDSIEDLEVTCTWK
LPTLAKFSPYLGQMINLRRLLLSHIHASSYISPEK
EEQYIAQFTSQFLSLQCLQALYVDSLFFLRGRLDQ
LLRHVMNPLETLSITNCRLSEGDVMHLSQSPSVSQ
LSVLSLSGVMLTDVSPEPLQALLERASATLQDLVF
DECGITDDQLLALLPSLSHCSQLTTLSFYGNSISI
SALQSLLQHLIGLSNLTHVLYPVPLESYEDIHGTL
HLERLAYLHARLRELLCELGRPSMVWLSANPCPHC
GDRTFYDPEPILCPCFMPN- Storage
 - Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
 
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					Supportive validation
					
									
				
				- Submitted by
 - Abnova Corporation (provider)
 - Main image
 
- Experimental details
 - Western Blot analysis of PRAME expression in transfected 293T cell line (H00023532-T02) by PRAME MaxPab polyclonal antibody.Lane 1: PRAME transfected lysate(57.90 KDa).Lane 2: Non-transfected lysate.
 
							
					Supportive validation
					
									
				
		- Submitted by
 - Abnova Corporation (provider)
 - Main image
 
- Experimental details
 - Immunofluorescence of purified MaxPab antibody to PRAME on HeLa cell. [antibody concentration 20 ug/ml]
 - Validation comment
 - Immunofluorescence
 - Protocol
 - Protocol