Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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Validation data
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- Product number
- PA5-98928 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SAV1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.31 mg/mL
- Storage
- -20°C or -80°C if preferred
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of SAV1 using a SAV1 Polyclonal antibody (Product # PA5-98928) at a concentration of 1.62 µg/mL. Positive WB detected in: 293T whole cell lysate, PC-3 whole cell lysate, A549 whole cell lysate, MCF-7 whole cell lysate, NIH/3T3 whole cell lysate. A secondary Goat polyclonal antibody to rabbit IgG was applied at a 1:50,000 dilution. Observed band size: 45 kDa.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of precipitated SAV1 from NIH/3T3 whole cell lysate using a SAV1 polyclonal antibody (Product # PA5-98928). An HRP-conjugated Protein G antibody was used as the secondary antibody (1:2000). Lane 1: Rabbit control IgG. Lane 2: NIH/3T3 whole cell lysate (500µg). Lane 3: NIH/3T3 whole cell lysate (20µg).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of SAV1 using a SAV1 Polyclonal antibody (Product # PA5-98928) at a concentration of 1.62 µg/mL. Positive WB detected in: 293T whole cell lysate, PC-3 whole cell lysate, A549 whole cell lysate, MCF-7 whole cell lysate, NIH/3T3 whole cell lysate. A secondary Goat polyclonal antibody to rabbit IgG was applied at a 1:50,000 dilution. Observed band size: 45 kDa.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of SAV1 in A549 cells using a SAV1 polyclonal antibody (Product # PA5-98928) at a dilution of 1:100. Alexa Fluor 488-congugated Goat Anti-Rabbit IgG(H+L) secondary antibody was used.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of SAV1 in paraffin embedded human endometrial cancer using a SAV1 polyclonal antibody (Product # PA5-98928) at a dilution of 1:100.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of SAV1 in paraffin embedded human kidney tissue using a SAV1 polyclonal antibody (Product # PA5-98928) at a dilution of 1:100.