Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Other assay [1]
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Validation data
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- Product number
- 61288 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CENP-B Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 10 µL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- CENP-B antibody (pAb) tested by Western blot. CENP-B detection by Western blot. The analysis was performed using 30 µg HeLa nuclear cell extract and CENP-B at a 1:500 dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- CENP-B antibody (pAb) tested by Immunoprecipitation. 10 µl of CENP-B antibody was used to immunoprecipitate CENP-B from 250 µg of HeLa nuclear cell extract (lane 2). 10 µl of rabbit IgG was used as a negative control (lane 1). The immunoprecipitated protein was detected by Western blotting using the CENP-B antibody at a dilution of 1:500.