H00023746-M04

antibody from Abnova Corporation

Targeting: AIPL1 LCA4

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

H00023746-M04

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00023746-M04, RRID:AB_1136886

Product name

AIPL1 monoclonal antibody (M04), clone 1E1

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a full-length recombinant AIPL1.

Antigen sequence

MDAALLLNVEGVKKTILHGGTGELPNFITGSRVIF
HFRTMKCDEERTVIDDSRQVGQPMHIIIGNMFKLE
VWEILLTSMRVHEVAEFWCDTIHTGVYPILSRSLR
QMAQGKDPTEWHVHTCGLANMFAYHTLGYEDLDEL
QKEPQPLVFVIELLQVDAPSDYQRETWNLSNHEKM
KAVPVLHGEGNRLFKLGRYEEASSKYQEAIICLRN
LQTKEKPWEVQWLKLEKMINTLILNYCQCLLKKEE
YYEVLEHTSDILRHHPGIVKAYYVRARAHAEVWNE
AEAKADLQKVLELEPSMQKAVRRELRLLENRMAEK
QEEERLRCRNMLSQGATQPPAEPPTEPPAQSSTEP
PAEPPTAPSAELSAGPPAEPATEPPPSPGHSLQH


Isotype

IgG

Antibody clone number

1E1

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of AIPL1 expression in transfected 293T cell line by AIPL1 monoclonal antibody (M04), clone 1E1.Lane 1: AIPL1 transfected lysate(43.9 KDa).Lane 2: Non-transfected lysate.

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged AIPL1 is 0.1 ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescence of monoclonal antibody to AIPL1 on HeLa cell . [antibody concentration 10 ug/ml]

Validation comment

Immunofluorescence

Protocol

Protocol

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunoperoxidase of monoclonal antibody to AIPL1 on formalin-fixed paraffin-embedded human placenta. [antibody concentration 3 ug/ml]

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Protocol

Protocol