PA5-85296

antibody from Invitrogen Antibodies

Targeting: GTPBP4 CRFG, FLJ10686, FLJ10690, NGB, NOG1

Antibody data

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Validation data

Product number: PA5-85296 - Provider product page
Provider: Invitrogen Antibodies
Product name: GTPBP4 Polyclonal Antibody
Antibody type: Polyclonal
Antigen: Recombinant full-length protein
Description: Keep as concentrated solution. Predicted reactivity: Mouse (91%), Rat (91%), Chicken (83%), Bovine (89%). Positive Control: 293T, A431, HeLa, HepG2, Neuro 2A, GL261, C8D30, NIH-3T3, BCL-1, C2C12, PC-12, Rat2. Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Reactivity: Human, Mouse, Rat
Host: Rabbit
Isotype: IgG
Vial size: 100 µL
Concentration: 1 mg/mL
Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

GTPBP4 protein structure - PA5-85296 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of GTPBP4 in A) PC-12 whole cell lysate, B) rat2 whole cell lysate using GTPBP4 polyclonal antibody (Product # PA5-85296) using 30 µg of sample at a dilution of 1:500. Prior to incubation with primary antibody, the sample was separated on 7.5% SDS-PAGE.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of GTPBP4 in A) Neuro2A whole cell lysate, B) GL261 whole cell lysate, C) C8D30 whole cell lysate, D) NIH-3T3 whole cell lysate, E) BCL-1 whole cell lysate, F) C2C12 whole cell lysate using GTPBP4 polyclonal antibody (Product # PA5-85296) using 30 µg of sample at a dilution of 1:500. Prior to incubation with primary antibody, the sample was separated on 7.5% SDS-PAGE.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: GTPBP4 Polyclonal Antibody detects GTPBP4 protein by western blot analysis. A 293T whole cell lysate/extract. B A431. C HeLa whole cell lysate/extract. D HepG2 whole cell lysate/extract.7.5 % SDS-PAGE. GTPBP4 Polyclonal Antibody (Product # PA5-85296) dilution: 1:1,000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: GTPBP4 Polyclonal Antibody detects GTPBP4 protein by western blot analysis. A. 30 µg Neuro2A whole cell lysate/extract. B. 30 µg GL261 whole cell lysate/extract. C. 30 µg C8D30 whole cell lysate/extract. D. 30 µg NIH-3T3 whole cell lysate/extract. E. 30 µg BCL-1 whole cell lysate/extract. F. 30 µg C2C12 whole cell lysate/extract.7.5 % SDS-PAGE. GTPBP4 Polyclonal Antibody (Product # PA5-85296) dilution: 1:500.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: GTPBP4 Polyclonal Antibody detects GTPBP4 protein by western blot analysis. A. 30 µg PC-12 whole cell lysate/extract. B. 30 µg Rat2 whole cell lysate/extract.7.5 % SDS-PAGE. GTPBP4 Polyclonal Antibody (Product # PA5-85296) dilution: 1:500.