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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [2]
- Immunohistochemistry [4]
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- Product number
- MA5-34719 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CD46 Recombinant Rabbit Monoclonal Antibody (JB25-49)
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide
- Description
- Positive Control: Siha, HUVEC, PC-3M, rat kidney tissue, human colon cancer tissue, human breast tissue, human placenta tissue.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Antibody clone number
- JB25-49
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles, store in dark
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of CD46 in Siha and PC-3M cell lysates. Samples were incubated with CD46 monoclonal antibody (Product # MA5-34719), at a dilution of 1:1000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of CD46 in PC-3M cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with CD46 monoclonal antibody (Product # MA5-34719), followed by DAPI (blue).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of CD46 in HUVEC cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with CD46 monoclonal antibody (Product # MA5-34719), followed by DAPI (blue).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of CD46 in paraffin-embedded rat kidney tissue. Samples were incubated with CD46 monoclonal antibody (Product # MA5-34719), and followed by hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of CD46 in paraffin-embedded human placenta tissue. Samples were incubated with CD46 monoclonal antibody (Product # MA5-34719), and followed by hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of CD46 in paraffin-embedded human colon cancer tissue. Samples were incubated with CD46 monoclonal antibody (Product # MA5-34719), and followed by hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of CD46 in paraffin-embedded human breast tissue. Samples were incubated with CD46 monoclonal antibody (Product # MA5-34719), and followed by hematoxylin.
