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Other assayAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Other assay [1]
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- Product number
- PA5-47042 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- c-Mpl Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant human Epo R is observed.
- Concentration
- 0.2 mg/mL
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Neutralization of c-Mpl in MO7e human megakaryocytic leukemic cell line. Samples were incubated in c-Mpl polyclonal antibody (Product # PA5-47042). Recombinant Human Thrombopoietin/Tpo stimulates proliferation in the MO7e human megakaryocytic leukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human Thrombopoietin/Tpo (10 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human Thrombopoietin R/Tpo R Antigen Affinity-purified Polyclonal Antibody. The ND50 is typically 0.5‚2.5 µg/mL.
