PA5-35325

antibody from Invitrogen Antibodies

Targeting: TMPRSS3 DFNB10, DFNB8

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-35325

Provider

Invitrogen Antibodies

Product name

TMPRSS3 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

400 µL

Concentration

0.5 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

Long non‑coding RNA EBLN3P promotes the recovery of the function of impaired spiral ganglion neurons by competitively binding to miR‑204‑5p and regulating TMPRSS3 expression.

Jiang W, Peng A, Chen Y, Pang B, Zhang Z
International journal of molecular medicine 2020 Jun;45(6):1851-1863

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of TMPRSS3 in Hela and 293 cell lysates (35 µg/lane) using a TMPRSS3 polyclonal antibody (Product # PA5-35325).

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of TMPRSS3 in Hela cells using a TMPRSS3 polyclonal antibody (Product # PA5-35325) followed by detection using a fluorescent conjugated secondary antibody (green). Nuclei were stained with Dapi (blue).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 3 lncRNA EBLN3P regulates the expression of TMPRSS3 through miR-204-5p in normal SGNs. (A) RT-qPCR was performed to detect the effects of lncRNA EBLN3P knockdown on the expression of EBLN3P in primary normal SGNs. (B) Luciferase activity was evaluated to observe the effect of lncRNA EBLN3P knockdown on the psiCHECK-2/TMPRSS3 wild-type 3'-UTR, psiCHECK-2/TMPRSS3 mutated 3'-UTR and empty 3'-UTR vectors (control). (C) RT-qPCR was performed to detect the effects of lncRNA EBLN3P knockdown on the mRNA expression of TMPRSS3 in primary normal SGNs. (D) Western blot analysis was performed to detect the effects of lncRNA EBLN3P knockdown on the protein expression of TMPRSS3 in primary normal SGNs. (E) RT-qPCR was performed to detect the effects of lncRNA EBLN3P overexpression on the expression of EBLN3P in primary normal SGNs. (F) Normal SGNs were treated with over-NC, over-lncRNA EBLN3P or anti-miR-204-5p + over-lncRNA EBLN3P, and the luciferase activity was evaluated. (G-I) Normal SGNs were treated with control over-NC, over-lncRNA EBLN3P or anti-miR-204-5p + over-lncRNA EBLN3P. RT-qPCR and western blot analysis was performed to detect the mRNA and protein expression of TMPRSS3 in primary normal SGNs. ** P