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- Product number
- PA5-35325 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- TMPRSS3 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 µL
- Concentration
- 0.5 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Long non‑coding RNA EBLN3P promotes the recovery of the function of impaired spiral ganglion neurons by competitively binding to miR‑204‑5p and regulating TMPRSS3 expression.
Jiang W, Peng A, Chen Y, Pang B, Zhang Z
International journal of molecular medicine 2020 Jun;45(6):1851-1863
International journal of molecular medicine 2020 Jun;45(6):1851-1863
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of TMPRSS3 in Hela cells using a TMPRSS3 polyclonal antibody (Product # PA5-35325) followed by detection using a fluorescent conjugated secondary antibody (green). Nuclei were stained with Dapi (blue).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry analysis of TMPRSS3 in HeLa cells. Samples were incubated in TMPRSS3 polyclonal antibody (Product # PA5-35325) followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 3 lncRNA EBLN3P regulates the expression of TMPRSS3 through miR-204-5p in normal SGNs. (A) RT-qPCR was performed to detect the effects of lncRNA EBLN3P knockdown on the expression of EBLN3P in primary normal SGNs. (B) Luciferase activity was evaluated to observe the effect of lncRNA EBLN3P knockdown on the psiCHECK-2/TMPRSS3 wild-type 3'-UTR, psiCHECK-2/TMPRSS3 mutated 3'-UTR and empty 3'-UTR vectors (control). (C) RT-qPCR was performed to detect the effects of lncRNA EBLN3P knockdown on the mRNA expression of TMPRSS3 in primary normal SGNs. (D) Western blot analysis was performed to detect the effects of lncRNA EBLN3P knockdown on the protein expression of TMPRSS3 in primary normal SGNs. (E) RT-qPCR was performed to detect the effects of lncRNA EBLN3P overexpression on the expression of EBLN3P in primary normal SGNs. (F) Normal SGNs were treated with over-NC, over-lncRNA EBLN3P or anti-miR-204-5p + over-lncRNA EBLN3P, and the luciferase activity was evaluated. (G-I) Normal SGNs were treated with control over-NC, over-lncRNA EBLN3P or anti-miR-204-5p + over-lncRNA EBLN3P. RT-qPCR and western blot analysis was performed to detect the mRNA and protein expression of TMPRSS3 in primary normal SGNs. ** P
