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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [4]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- PA5-31441 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ITM2B Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: HepG2, HCT116, mouse brain, rat brain.
- Concentration
- 0.81 mg/mL
Submitted references First identification of ITM2B interactome in the human retina.
Wohlschlegel J, Argentini M, Michiels C, Letellier C, Forster V, Condroyer C, He Z, Thuret G, Zeitz C, Léger T, Audo I
Scientific reports 2021 Aug 26;11(1):17210
Scientific reports 2021 Aug 26;11(1):17210
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of ITM2B using 30 µg of A) HepG2 and B) HCT116 lysate. Samples were loaded onto a 12% SDS-PAGE gel and probed with an ITM2B polyclonal antibody (Product # PA5-31441) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of ITM2B using 50 µg mouse brain lysate. Samples were loaded onto a 12% SDS-PAGE gel and probed with an ITM2B polyclonal antibody (Product # PA5-31441) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of ITM2B was performed by separating 50 µg of rat tissue extract by 12% SDS-PAGE. Proteins were transferred to a membrane and probed with a ITM2B Polyclonal Antibody (Product # PA5-31441) at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using ITM2B Polyclonal Antibody (Product # PA5-31441). Whole cell extract (30 µg) was separated by 12% SDS-PAGE, and the membrane was blotted with ITM2B Polyclonal Antibody (Product # PA5-31441) diluted at 1:5,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of ITM2B in methanol-fixed HeLa cells using an ITM2B polyclonal antibody (Product # PA5-31441) at a 1:200 dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- ITM2B Polyclonal Antibody detects ITM2B protein at Golgi apparatus on mouse esophagus by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain stem. ITM2B Polyclonal Antibody (Product # PA5-31441) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
