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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
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- Product number
- AP8135b - Provider product page
- Provider
- Abcepta
- Proper citation
- Abgent Cat#AP8135b, RRID:AB_2163027
- Product name
- PFKP Antibody (C-term)
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Purified Rabbit Polyclonal Antibody (Pab)
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 µl
- Concentration
- 2 mg/ml
- Storage
- Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Submitted references Energy management by enhanced glycolysis in G1-phase in human colon cancer cells in vitro and in vivo.
Nuclear targeting of 6-phosphofructo-2-kinase (PFKFB3) increases proliferation via cyclin-dependent kinases.
Cytosolic action of thyroid hormone leads to induction of hypoxia-inducible factor-1alpha and glycolytic genes.
Bao Y, Mukai K, Hishiki T, Kubo A, Ohmura M, Sugiura Y, Matsuura T, Nagahata Y, Hayakawa N, Yamamoto T, Fukuda R, Saya H, Suematsu M, Minamishima YA
Molecular cancer research : MCR 2013 Sep;11(9):973-85
Molecular cancer research : MCR 2013 Sep;11(9):973-85
Nuclear targeting of 6-phosphofructo-2-kinase (PFKFB3) increases proliferation via cyclin-dependent kinases.
Yalcin A, Clem BF, Simmons A, Lane A, Nelson K, Clem AL, Brock E, Siow D, Wattenberg B, Telang S, Chesney J
The Journal of biological chemistry 2009 Sep 4;284(36):24223-32
The Journal of biological chemistry 2009 Sep 4;284(36):24223-32
Cytosolic action of thyroid hormone leads to induction of hypoxia-inducible factor-1alpha and glycolytic genes.
Moeller LC, Dumitrescu AM, Refetoff S
Molecular endocrinology (Baltimore, Md.) 2005 Dec;19(12):2955-63
Molecular endocrinology (Baltimore, Md.) 2005 Dec;19(12):2955-63
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Supportive validation
- Submitted by
- Abcepta (provider)
- Main image
- Experimental details
- PFKP Antibody (D769) (Cat. #AP8135b) western blot analysis in T47D cell line lysates (35ug/lane).This demonstrates the PFKP antibody detected the PFKP protein (arrow).
- Primary Ab dilution
- 1:1000
- Submitted by
- Abcepta (provider)
- Main image
- Experimental details
- "Fibroblasts were cultured for 2 d in DMEM supplemented with 10% TH-depleted bovine serum. They were then treated with 2 nM T3 in the absence or presence of either LY294002 (LY, 50 M) or PD98059 (PD, 50 M), added 1 h before the addition of T3. Cells were harvested 24 h after T3 treatment. Whole-cell lysates, obtained from two separately treated culture dishes for each treatment, were subjected to WB with anti-PFKP. (Mol. Endocrinol. 2005 Dec 01;19(12):2955-2963)"
- Primary Ab dilution
- 1:1000
Supportive validation
- Submitted by
- Abcepta (provider)
- Main image
- Experimental details
- "Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma."
- Primary Ab dilution
- 1:50~100
