TA503985

antibody from OriGene

Targeting: PFKP PFK-C, PFKF

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

TA503985

Provider

OriGene

Proper citation

OriGene Cat#TA503985, RRID:AB_11126876

Product name

PFKP mouse monoclonal antibody, clone OTI1A11 (formerly 1A11)

Antibody type

Monoclonal

Description

PFKP mouse monoclonal antibody, clone OTI1A11 (formerly 1A11)

Host

Mouse

Conjugate

Unconjugated

Epitope

PFKP

Isotype

IgG

Antibody clone number

OTI1A11

Vial size

100 µl

Concentration

0.44 mg/ml

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-PFKP monoclonal antibody.

Validation comment

WB

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PFKP (RC200673, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PFKP.

Validation comment

WB

Immunocytochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Anti-PFKP mouse monoclonal antibody (TA503985) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PFKP(RC200673).

Validation comment

IF

Immunohistochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-PFKP mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503985)

Validation comment

IHC

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-PFKP mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503985)

Validation comment

IHC

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-PFKP mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503985)

Validation comment

IHC

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-PFKP mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503985)

Validation comment

IHC

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-PFKP mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503985)

Validation comment

IHC

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-PFKP mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503985)

Validation comment

IHC

Flow cytometry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Flow cytometric Analysis of Jurkat cells, using anti-PFKP antibody(TA503985),(Red), compared to a nonspecific negative control antibody,(Blue).

Validation comment

FC

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Flow cytometric Analysis of Hela cells, using anti-PFKP antibody(TA503985),(Red), compared to a nonspecific negative control antibody,(Blue).

Validation comment

FC