Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [4]
- Immunohistochemistry [7]
- Flow cytometry [1]
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Validation data
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- Product number
- NBP2-01539 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal PFKP Antibody
- Antibody type
- Monoclonal
- Description
- Affinity purified.
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 0.48 mg/ml
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: PFKP Antibody (1D6) [NBP2-01539] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PFKP (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PFKP.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: PFKP Antibody (1D6) [NBP2-01539] Analysis of extracts (35ug) from 9 different cell lines by using anti-PFKP monoclonal antibody.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: PFKP Antibody (OTI1D6) [NBP2-01539] - Analysis of extracts (10ug) from 9 Human tissues by using anti-PFKP monoclonal antibody at 1:200 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: colon).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: PFKP Antibody (OTI1D6) [NBP2-01539] - Equivalent amounts of cell lysates (10 ug per lane) of wild-type HeLa cellsand PFKP-Knockout HeLa cells were separated by SDS-PAGE and immunoblotted with anti-PFKP monoclonal antibody (1:500). Then the blotted membrane was stripped and reprobed with anti-PCNA antibody as a loading control.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: PFKP Antibody (1D6) [NBP2-01539] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-PFKP mouse monoclonal antibody.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: PFKP Antibody (1D6) [NBP2-01539] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-PFKP mouse monoclonal antibody.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: PFKP Antibody (1D6) [NBP2-01539] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-PFKP mouse monoclonal antibody.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: PFKP Antibody (1D6) [NBP2-01539] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-PFKP mouse monoclonal antibody.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: PFKP Antibody (1D6) [NBP2-01539] - Staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-PFKP mouse monoclonal antibody.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: PFKP Antibody (1D6) [NBP2-01539] - Staining of paraffin-embedded Carcinoma of Human lung tissue using anti-PFKP mouse monoclonal antibody.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: PFKP Antibody (1D6) [NBP2-01539] - Staining of paraffin-embedded Human endometrium tissue using anti-PFKP mouse monoclonal antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: PFKP Antibody (1D6) [NBP2-01539] - Analysis of Jurkat cells, using anti-PFKP antibody, (Red), compared to a nonspecific negative control antibody (Blue).