MA5-50776

antibody from Invitrogen Antibodies

Targeting: PSMC6 p42

Western blot (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-50776

Provider

Invitrogen Antibodies

Product name

PSMC6 Recombinant Rabbit Monoclonal Antibody (PSH0-12)

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Description

Positive control: Hela cell lysate, 293T cell lysate, SH-SY5Y cell lysate, HepG2 cell lysate, PC-12 cell lysate, NIH/3T3 cell lysate, mouse kidney tissue lysate, rat kidney tissue lysate, rat brain tissue lysate, human testis tissue, SH-SY5Y, NIH/3T3, PC-12. Predicted band size: 44 kDa Subcellular Location: Cytoplasm, Nucleus.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Antibody clone number

PSH0-12

Vial size

100 μL

Concentration

1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of PSMC6 in paraffin-embedded human testis tissue. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with PSMC6 recombinant monoclonal antibody (Product # MA5-50776) with a dilution of 1:400 for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen, and tissues were counterstained with hematoxylin and mounted with DPX.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry analysis of PSMC6 in NIH/3T3 cells. The cells were fixed, permeabilized, and then stained with PSMC6 recombinant monoclonal antibody (Product # MA5-50776) at a dilution of 1 µg/mL (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at 4℃ for an hour, cells were stained with secondary antibody iFluor™ 488 conjugate-Goat anti-Rabbit IgG at a dilution of 1:1,000 for 30 minutes at 4℃. Unlabeled sample was used as a control (cells without incubation with primary antibody; black).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry analysis of PSMC6 in SH-SY5Y cells. The cells were fixed, permeabilized, and then stained with PSMC6 recombinant monoclonal antibody (Product # MA5-50776) at a dilution of 1 µg/mL (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at 4℃ for an hour, cells were stained with secondary antibody iFluor™ 488 conjugate-Goat anti-Rabbit IgG at a dilution of 1:1,000 for 30 minutes at 4℃. Unlabeled sample was used as a control (cells without incubation with primary antibody; black).