Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PA5-30672 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- RCBTB1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: NT2D1, IMR32, U87-MG, MCF-7, mouse brain. Predicted reactivity: Human (99%), Mouse (93%), Rat (93%), Rhesus Monkey (97%), Bovine (95%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.46 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using RCBTB1 Polyclonal Antibody (Product # PA5-30672). Sample (50 µg of whole cell lysate). Lane A: mouse brain. 7.5% SDS PAGE. RCBTB1 Polyclonal Antibody (Product # PA5-30672) diluted at 1:2,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using RCBTB1 Polyclonal Antibody (Product # PA5-30672). Sample (30 µg of whole cell lysate). Lane A: U87-MG. 12% SDS PAGE. RCBTB1 Polyclonal Antibody (Product # PA5-30672) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of RCBTB1 in paraformaldehyde-fixed MCF-7 cells using a RCBTB1 polyclonal antibody (Product # PA5-30672) at a 1:200 dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded J5 xenograft, using RCBTB1 (Product # PA5-30672) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
