AF7788

antibody from Novus Biologicals

Targeting: PTGIS CYP8A1, PGIS

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

AF7788

Provider

Novus Biologicals

Product name

Sheep Polyclonal Prostaglandin I2 Synthase Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified. Detects human Prostaglandin I2 Synthase in direct ELISAs and Western blots.

Reactivity

Human

Host

Sheep

Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Detection of Human Prostaglandin I2 Synthase by Western Blot. Western blot shows lysates of SK-OV-3 human ovarian adenocarcinoma cell line, human ovary tissue, human prostate tissue, and human heart tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human Prostaglandin I2 Synthase Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7788) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Prostaglandin I2 Synthase at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.