Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [5]
- ELISA [1]
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Validation data
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- Product number
- H00001159-M04 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CKMT1B Monoclonal Antibody (2C8)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- Sequence of this protein is as follows: GVHIKLPLLS KDSRFPKILE NLRLQKRGTG GVDTAATGGV FDISNLDRLG KSEVELVQLV IDGVNYLIDC ERRLERGQDI RIPTPVIHTK H
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 2C8
- Vial size
- 100 µg
- Concentration
- See Label
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of CKMT1B over-expressed 293 cell line, cotransfected with CKMT1B Validated Chimera RNAi (Cat # H00001159-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with CKMT1B monoclonal antibody (M04), clone 2C8 (Cat # H00001159-M04). GAPDH ( 36.1 kDa ) used as specificity and loading control.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of CKMT1B expression in transfected 293T cell line by CKMT1B monoclonal antibody (M04), clone 2C8.Lane 1: CKMT1B transfected lysate(47 KDa).Lane 2: Non-transfected lysate.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of CKMT1B over-expressed 293 cell line, cotransfected with CKMT1B Validated Chimera RNAi (Cat # H00001159-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with CKMT1B monoclonal antibody (M04), clone 2C8 (Cat # H00001159-M04). GAPDH ( 36.1 kDa ) used as specificity and loading control.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- CKMT1B monoclonal antibody (M04), clone 2C8. Western Blot analysis of CKMT1B expression in A-431 (Cat # L015V1).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of CKMT1B expression in transfected 293T cell line by CKMT1B monoclonal antibody (M04), clone 2C8.Lane 1: CKMT1B transfected lysate(47 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged CKMT1B is approximately 0.1 ng/mL as a capture antibody.