GTX60197

antibody from GeneTex

Targeting: CD274 B7-H, B7-H1, B7H1, PD-L1, PDCD1LG1, PDL1

ELISA (Supportive data in Antibodypedia)

Immunohistochemistry (Recommended by provider)

Antibody Data

Product number

GTX60197

Provider

GeneTex

Product name

PD-L1 antibody

Antibody type

Polyclonal

Antigen

KLH conjugated synthetic peptide derived from mouse B7-H1 C-terminus

Host

Rabbit

Isotype

IgG

Vial size

100µg

Concentration

1 mg/ml

Storage

Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

ELISA

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Antigen: GTX60197, 0.2ug/100ul Primary: Antiserum 1:500, 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000 Secondary: HRP conjugated Goat Anti-Rabbit IgG (GTX60197) at 1: 5000; TMB staining Read the data in MicroplateReader by 450nm. 

Validation comment

ELISA

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

ELISA analysis with 0.2ug/100ul antigen and serially diluted B7-H1 antibody (1:500, 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000).

Validation comment

ELISA