GTX117446

antibody from GeneTex

Targeting: CD274 B7-H, B7-H1, B7H1, PD-L1, PDCD1LG1, PDL1

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Recommended by provider)

Antibody Data

Product number

GTX117446

Provider

GeneTex

Proper citation

GeneTex Cat#GTX117446, RRID:AB_10615812

Product name

PD-L1 antibody [N1C2]

Antibody type

Polyclonal

Antigen

Recombinant protein encompassing a sequence within the center region of human PD-L1. The exact sequence is proprietary.

Reactivity

Bovine, Simian

Host

Rabbit

Conjugate

Unconjugated

Isotype

IgG

Vial size

25µl, 100µl

Concentration

1 mg/ml

Storage

Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

References

Submitted references

Predictive value of PD-L1 based on mRNA level in the treatment of stage IV melanoma with ipilimumab.

Brüggemann C, Kirchberger MC, Goldinger SM, Weide B, Konrad A, Erdmann M, Schadendorf D, Croner RS, Krähenbühl L, Kähler KC, Hafner C, Leisgang W, Kiesewetter F, Dummer R, Schuler G, Stürzl M, Heinzerling L
Journal of cancer research and clinical oncology 2017 Oct;143(10):1977-1984

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Glycosylation and stabilization of programmed death ligand-1 suppresses T-cell activity.

Li CW, Lim SO, Xia W, Lee HH, Chan LC, Kuo CW, Khoo KH, Chang SS, Cha JH, Kim T, Hsu JL, Wu Y, Hsu JM, Yamaguchi H, Ding Q, Wang Y, Yao J, Lee CC, Wu HJ, Sahin AA, Allison JP, Yu D, Hortobagyi GN, Hung MC
Nature communications 2016 Aug 30;7:12632

Western blot

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Sample (30 ug of whole cell lysate) A: Hela 12% SDS PAGE GTX117446 diluted at 1:1000

Validation comment

WB