Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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Validation data
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- Product number
- ARG55930 - Provider product page
- Provider
- Arigo
- Product name
- anti-CD274 / PD-L1 antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide around 17 aa (Center) of Human CD274 / PD-L1
- Description
- Affinity purification with immunogen.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 50 µg
- Concentration
- 1 mg/ml
- Storage
- For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening.
- Handling
- The antibody solution should be gently mixed before use.
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Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Western blot: Raji cell lysate stained with ARG55930 anti-CD274 / PD-L1 antibody at 1) 0.5 and 2) 1 µg/ml dilution.
Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Immunofluorescence: Human Heart cells stained with ARG55930 anti-CD274 / PD-L1 antibody at 20 µg/ml dilution.
Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Immunohistochemistry: Human tonsil tissue stained with ARG55930 anti-CD274 / PD-L1 antibody at 5 µg/ml.
Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Flow Cytometry: A-20 cells stained with ARG55930 anti-CD274 / PD-L1 antibody at 0.5 µg/ml dilution (red); Isotype control (green).