Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [11]
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Validation data
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- Product number
- UM800120 - Provider product page
- Provider
- OriGene
- Product name
- PD-L1/CD274 mouse monoclonal antibody, clone UMAB228
- Antibody type
- Monoclonal
- Description
- PD-L1/CD274 mouse monoclonal antibody, clone UMAB228
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- CD274
- Isotype
- IgG
- Antibody clone number
- UMAB228
- Vial size
- 100 µl
- Concentration
- 1.00 mg/ml
Submitted references Assessment of programmed cell death ligand-1 expression with multiple immunohistochemistry antibody clones in non-small cell lung cancer.
Pang C, Yin L, Zhou X, Lei C, Tong R, Huang M, Gong Y, Ding Z, Xue J, Zhu J, Wang Y, Ren L, Zhou L, Wang J, Peng F, Zhou Q, Lu Y
Journal of thoracic disease 2018 Feb;10(2):816-824
Journal of thoracic disease 2018 Feb;10(2):816-824
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (35ug) from 5 different cancer cell lines by using anti-CD274 monoclonal antibody .(1:500)
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded SK-MEL-28 cell pellets using anti-CD274 (PD-L1) mouse monoclonal antibody (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH9.0) at 120°C for 2.5 min, UM800120)(1:300)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded NCI-H2228 cell pellets using anti-CD274 (PD-L1) mouse monoclonal antibody (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH9.0) at 120°C for 2.5 min, UM800120)(1:300)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded MDA-MB-231 cell pellets using anti-CD274 (PD-L1) mouse monoclonal antibody (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH9.0) at 120°C for 2.5 min, UM800120)(1:300)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded MCF7 (PD-L1 Negative cell line) cell pellets using anti-CD274 (PD-L1) mouse monoclonal antibody (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH9.0) at 120°C for 2.5 min, UM800120)(1:300)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded LoVo (PD-L1 Negative cell line) cell pellets using anti-CD274 (PD-L1) mouse monoclonal antibody (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH9.0) at 120°C for 2.5 min, UM800120)(1:300)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-CD274 (PD-L1) mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH9.0) at 120°C for 2.5 min, UM800120)(1:300)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-CD274 (PD-L1) mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH9.0) at 120°C for 2.5 min, UM800120)(1:300)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-CD274 (PD-L1) mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH9.0) at 120°C for 2.5 min, UM800120)(1:300)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-CD274 (PD-L1) mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH9.0) at 120°C for 2.5 min, UM800120)(1:300)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Carcinoma of Human Esophagus using anti-CD274 (PD-L1) mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH9.0) at 120°C for 2.5 min, UM800120)(1:300)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue tissue using anti-CD274 (PD-L1) mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH9.0) at 120°C for 2.5 min, UM800120)(1:300)
- Validation comment
- IHC