TA810750

antibody from OriGene

Targeting: CD274 B7-H, B7-H1, B7H1, PD-L1, PDCD1LG1, PDL1

Western blot (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

TA810750

Provider

OriGene

Product name

CD274 mouse monoclonal antibody,clone OTI20G10

Antibody type

Monoclonal

Description

CD274 mouse monoclonal antibody,clone OTI20G10

Host

Mouse

Conjugate

Unconjugated

Epitope

CD274

Isotype

IgG

Antibody clone number

OTI20G10

Vial size

100 µl

Concentration

1.00mg/ml

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD274 (RC213071, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD274.(1:500)

Validation comment

WB

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Western blot analysis of extracts (35ug) from 9 different cancer cell lines by using anti-CD274 monoclonal antibody .(1:500)

Validation comment

WB

Flow cytometry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Living HEK293T cells transfected with either RC213071 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-CD274 antibody(TA810750), and then analyzed by flow cytometry.(1:100)

Validation comment

FC

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Flow cytometric Analysis of HCC78 cells, using anti-PDL1 antibody(TA810750),(Red), compared to isotype control,(green), and negative control (PBS),(Blue).(1:100)

Validation comment

FC