PAB12985

antibody from Abnova Corporation

Targeting: CD274 B7-H, B7-H1, B7H1, PD-L1, PDCD1LG1, PDL1

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB12985

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#PAB12985, RRID:AB_10547419

Product name

CD274 polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against synthetic peptide of CD274.

Isotype

IgG

Storage

Store at 4°C for three months. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

References

Submitted references

Immune accessory functions of human endothelial cells are modulated by overexpression of B7-H1 (PDL1).

LaGier AJ, Pober JS
Human immunology 2006 Aug;67(8):568-78

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Function and regulation of MHC class II molecules in T-lymphocytes: of mice and men.

Holling TM, Schooten E, van Den Elsen PJ
Human immunology 2004 Apr;65(4):282-90

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Induced expression of PD-1, a novel member of the immunoglobulin gene superfamily, upon programmed cell death.

Ishida Y, Agata Y, Shibahara K, Honjo T
The EMBO journal 1992 Nov;11(11):3887-95

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of CD274 in Raji cell lysate with CD274 polyclonal antibody (Cat # PAB12985) at (A) 0.5 and (B) 1 ug/mL .

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemistry of CD274 in human heart tissue with CD274 polyclonal antibody (Cat # PAB12985) at 2.5 ug/mL .

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)