48-5983-42

antibody from Invitrogen Antibodies
Targeting: CD274 B7-H, B7-H1, B7H1, PD-L1, PDCD1LG1, PDL1
Provider product page for 48-5983-42

Antibody data

Product number
48-5983-42 - Provider product page
Provider
Invitrogen Antibodies
Product name
CD274 (PD-L1, B7-H1) Monoclonal Antibody (MIH1), eFluor™ 450, eBioscience™
Antibody type
Monoclonal
Antigen
Other
Description
Description: The MIH1 monoclonal antibody reacts with human B7-H1, also known as PD-L1. B7-H1, a member of the B7 family, has a predicted molecular weight of approximately 40 kDa and belongs to the Ig superfamily. B7-H1 is expressed on a majority of leukocytes. B7-H1 is a ligand for PD-1. Interaction of PD-1 with either PD-L1 (B7-H1) or PD-L2 (B7-DC) results in inhibition of T and B cell responses. MIH1 is reported to be a blocking antibody. Applications Reported: This MIH1 antibody has been reported for use in flow cytometric analysis. Applications Tested: This MIH1 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.5 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. eFluor® 450 is an alternative to Pacific Blue®. eFluor® 450 emits at 445 nm and is excited with the Violet laser (405 nm). Please make sure that your instrument is capable of detecting this fluorochome. Excitation: 405 nm; Emission: 445 nm; Laser: Violet Laser. Filtration: 0.2 µm post-manufacturing filtered.
Reactivity
Human
Host
Mouse
Isotype
IgG
Antibody clone number
MIH1
Vial size
100 Tests
Concentration
5 µL/Test
Storage
4° C, store in dark, DO NOT FREEZE!
CD274 protein structure - 48-5983-42 shown in red.