Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [2]
- Immunohistochemistry [1]
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Validation data
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- Product number
- R20329 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- Recombinant PD-L1 Antibody / B7-H1 / CD274 / C-Terminal
- Antibody type
- Monoclonal
- Description
- Protein A purified from animal origin-free supernatant
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Antibody clone number
- RM311
- Vial size
- 100 µl
- Storage
- Store the recombinant PD-L1 antibody at -20°C.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of human H1975 (lung cancer line), DU145 (prostate cancer line) and LNCaP (prostate cancer line) cell lysate with recombinant PD-L1 antibody at 1:100 dilution. Predicted molecular weight: 34-70 kDa depending on glycosylation level.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IF / ICC testing of human H1975 cells (lung cancer line) with recombinant PD-L1 antibody at 1:100 dilution (red). Nuclei have been labeled with DAPI (blue).
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- ICC testing of formalin fixed and paraffin embedded human H1975 cells (lung cancer line) with recombinant PD-L1 antibody at 1:300 dilution. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC testing of formalin fixed and paraffin embedded human tonsil tissue with recombinant PD-L1 antibody at 1:300 dilution. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.