LS-C669076

antibody from LSBio

Targeting: CD274 B7-H, B7-H1, B7H1, PD-L1, PDCD1LG1, PDL1

Western blot (Enhanced validation)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Enhanced validation)

Antibody Data

Product number

LS-C669076

Provider

LSBio

Product name

CD274 / B7-H1 / PD-L1 Antibody (clone 4F2) LS-C669076

Antibody type

Monoclonal

Description

Protein A purified

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

4F2

Storage

Store at 4°C for up 3 months. Store at -20°C for up to 1 year. Avoid freeze/thaw cycles.

Western blot

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Western blot analysis of PD-L1 in overexpressing HEK293 cells PD-L1 antibody at 0.25 and 0.5 ug/ml

Immunocytochemistry

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Immunocytochemistry of PD-L1 in transfected HEK293 cells with PD-L1 antibody at 1 ug/mL. Lower left: Immunocytochemistry in transfected HEK293 cells with control mouse IgG antibody at 1 ug/mL.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Immunocytochemistry of PD-L1 in transfected HEK293 cells with PD-L1 antibody at 1 ug/mL. Lower left: Immunocytochemistry in transfected HEK293 cells with control mouse IgG antibody at 1 ug/mL.

Immunohistochemistry

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Immunohistochemistry of PD-L1 in human tonsil tissue with PD-L1 antibody at 5 ug/mL.

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Immunohistochemistry of PD-L1 in human stomach carcinoma tissue with PD-L1 antibody at 5 ug/mL.

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Immunohistochemistry of PD-L1 in human stomach carcinoma tissue with PD-L1 antibody at 5 ug/mL.

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Immunohistochemistry of PD-L1 in human tonsil tissue with PD-L1 antibody at 5 ug/mL.

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Immunohistochemistry of PD-L1 in human stomach carcinoma tissue with PD-L1 antibody at 5 ug/mL.