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LearnSTJ130023
antibody from St John's Laboratory
Targeting: CD274
B7-H, B7-H1, B7H1, PD-L1, PDCD1LG1, PDL1
Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Other assay [2]
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- Product number
- STJ130023 - Provider product page
- Provider
- St John's Laboratory
- Product name
- Anti-PD-L1 antibody [8C6] (STJ130023)
- Antibody type
- Monoclonal
- Description
- Rabbit monoclonal anti-PD-L1 antibody is suitable for use in ELISA, Western Blot and Flow Cytometry.
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Antigen sequence
NA- Epitope
- NA
- Isotype
- IgG
- Antibody clone number
- NA
- Vial size
- NA
- Concentration
- NA
- Storage
- Store Anti-PD-L1 Antibody at-20°C, and avoid repeated freeze-thaw cycles.
- Handling
- NA
No comments: Submit comment
Supportive validation
- Submitted by
- St John's Laboratory (provider)
- Main image
- Experimental details
- Western blot analysis of PD-L1 transfected, and non transfected 293T cells, using Anti-PD-L1 monoclonal antibody (STJ130023) [8C6] at 1:1000 dilution.
- Sample type
- NA
- Validation comment
- NA
- Primary Ab dilution
- NA
- Other comments
- NA
- Secondary Ab
- NA
- Secondary Ab dilution
- NA
- Protocol
- NA
Supportive validation
Supportive validation
- Submitted by
- St John's Laboratory (provider)
- Main image
- Experimental details
- Using Anti-PD-L1 monoclonal antibody (STJ130023), flow cytometry analysis of PD-L1 expressing 293F cells. Cells were stained with either control IgG or Anti-PD-L1 monoclonal antibody (STJ130023) [8C6] at 1:100 dilution
- Sample type
- NA
- Validation comment
- NA
- Primary Ab dilution
- NA
- Other comments
- NA
- Secondary Ab
- NA
- Secondary Ab dilution
- NA
- Protocol
- NA
Supportive validation
- Submitted by
- St John's Laboratory (provider)
- Main image
- Experimental details
- Using Anti-PD-L1 monoclonal antibody (STJ130023), inhibition assay results following 1 hour incubation of recombinantly expressed PD-1-lined ELISA plates with either: serial diluted rabbit monoclonal anti-PD-L1 antibody [8C6] (STJ130023), or a monoclonal control antibody.
- Sample type
- NA
- Validation comment
- NA
- Primary Ab dilution
- NA
- Other comments
- NA
- Secondary Ab
- NA
- Secondary Ab dilution
- NA
- Protocol
- NA
