LS-C742091

antibody from LSBio

Targeting: CD274 B7-H, B7-H1, B7H1, PD-L1, PDCD1LG1, PDL1

ELISA (Supportive data in Antibodypedia)

Antibody Data

Product number

LS-C742091

Provider

LSBio

Product name

CD274 / B7-H1 / PD-L1 Antibody (clone PDL1.D1) LS-C742091

Antibody type

Monoclonal

Description

Protein A affinity chromatography

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

PDL1.D1

Storage

Long term: store at -20°C for up to 2 years. Reconstituted: store at 2°C to 8°C for up to 3 weeks or at -20°C for up to 1 year. Avoid freeze/thaw cycles.

ELISA

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

ELISA blocking of human PD-L1 antibody PDL1.D1 against Human PD-1 recombinant protein (PD 1 Fc Chimera, Human) binding with Human PD-L1 recombinant protein (PD L1 Fc Chimera, Human). Coating antigen: PD-L1-Fc, 1µg/ml. PD-1-Fc final concentration: 0.5µg /ml PD-L1 antibody dilution start from 50µg/ml, IC50= 0.5µg/ml

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

ELISA binding of human PD-L1 antibody PDL1.D1 with Human PD-L1 recombinant protein (PD L1 Fc Chimera, Human). Coating antigen: PD-L1-Fc, 1µg/ml. PD-L1 antibody dilution start from 1000ng/ml, EC50= 4.4 ng/ml

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

ELISA blocking of human PD-L1 antibody PDL1.D1 against Human PD-1 recombinant protein (PD 1 Fc Chimera, Human) binding with Human PD-L1 recombinant protein (PD L1 Fc Chimera, Human). Coating antigen: PD-L1-Fc, 1µg/ml. PD-1-Fc final concentration: 0.5µg /ml PD-L1 antibody dilution start from 50µg/ml, IC50= 0.5µg/ml

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

ELISA binding of human PD-L1 antibody PDL1.D1 with Human PD-L1 recombinant protein (PD L1 Fc Chimera, Human). Coating antigen: PD-L1-Fc, 1µg/ml. PD-L1 antibody dilution start from 1000ng/ml, EC50= 4.4 ng/ml

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

ELISA blocking of human PD-L1 antibody PDL1.D1 against Human PD-1 recombinant protein (PD 1 Fc Chimera, Human) binding with Human PD-L1 recombinant protein (PD L1 Fc Chimera, Human). Coating antigen: PD-L1-Fc, 1µg/ml. PD-1-Fc final concentration: 0.5µg /ml PD-L1 antibody dilution start from 50µg/ml, IC50= 0.5µg/ml

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

ELISA binding of human PD-L1 antibody PDL1.D1 with Human PD-L1 recombinant protein (PD L1 Fc Chimera, Human). Coating antigen: PD-L1-Fc, 1µg/ml. PD-L1 antibody dilution start from 1000ng/ml, EC50= 4.4 ng/ml