V3955

antibody from NSJ Bioreagents

Targeting: CD274 B7-H, B7-H1, B7H1, PD-L1, PDCD1LG1, PDL1

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Protein array (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

V3955

Provider

NSJ Bioreagents

Product name

PD-L1 Antibody / B7-H1 / CD274

Antibody type

Monoclonal

Description

This highly specific PD-L1 antibody is suitable for use in ELISA/Western blot/Flow cytometry/Immunofluorescence/Immunohistochemistry applications with human and mouse samples.

Reactivity

Human, Mouse

Host

Mouse

Conjugate

Unconjugated

Antibody clone number

PDL1/2746

Vial size

20 ug (with BSA and sodium azide), 100 ug (with BSA and sodium azide), 100 ug (without BSA or sodium azide), 7 ml IHC only format (if applicable)

Concentration

0.2 mg/ml, 1 mg/ml

Storage

Store the PD-L1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Western blot

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of mouse and human cell lysates with PD-L1 antibody (PDL1/2746). Expected molecular weight ~34 kDa (unmodified), 45-70 kDa (glycosylated).

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of human cell lysates with PD-L1 antibody (PDL1/2746). Expected molecular weight ~34 kDa (unmodified), 45-70 kDa (glycosylated).

Immunocytochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Immunofluorescent staining of human Jurkat cells with PD-L1 antibody (clone PDL1/2746) and a CF488 labeled secondary (green). Nuclei were counterstained with Reddot (red).

Immunohistochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC testing of FFPE lung SCC with PD-L1 antibody (clone PDL1/2746). HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC testing of FFPE breast carcinoma with PD-L1 antibody (clone PDL1/2746). HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC testing of FFPE cervical carcinoma with PD-L1 antibody (clone PDL1/2746). HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC testing of FFPE basal cell carcinoma with PD-L1 antibody (clone PDL1/2746). HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.

Flow cytometry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

FACS testing of human Jurkat cells with PD-L1 antibody; Blue=cells alone, Red=isotype control, Orange= PD-L1 antibody.

Protein array

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using PD-L1 antibody (clone PDL1/2746). These results demonstrate the foremost specificity of the PDL1/2746 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SDs) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SDs) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

Other assay

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

SDS-PAGE analysis of purified, BSA-free PD-L1 antibody (clone PDL1/2746) as confirmation of integrity and purity.