Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [2]
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Validation data
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- Product number
- PA5-27758 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- MCCC2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: 293T, A431, H1299, HepG2, mouse liver. Predicted reactivity: Mouse (88%), Rat (88%), Zebrafish (84%), Xenopus laevis (84%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Ataxin-2 (Atxn2)-Knock-Out Mice Show Branched Chain Amino Acids and Fatty Acids Pathway Alterations.
Meierhofer D, Halbach M, Şen NE, Gispert S, Auburger G
Molecular & cellular proteomics : MCP 2016 May;15(5):1728-39
Molecular & cellular proteomics : MCP 2016 May;15(5):1728-39
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using MCCC2 Polyclonal Antibody (Product # PA5-27758). Sample (30 µg of whole cell lysate). Lane A: Hep G2 . 7.5% SDS PAGE. MCCC2 Polyclonal Antibody (Product # PA5-27758) diluted at 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using MCCC2 Polyclonal Antibody (Product # PA5-27758). Sample (50 µg of whole cell lysate). Lane A: mouse liver . 7.5% SDS PAGE. MCCC2 Polyclonal Antibody (Product # PA5-27758) diluted at 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of MCCC2 was performed in paraffin-embedded mouse cervix tissue using MCCC2 Polyclonal Antibody (Product # PA5-27758) at a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded SW480 xenograft, using MCCC2 (Product # PA5-27758) antibody at 1:100 dilution. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.