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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [2]
- Immunohistochemistry [2]
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- Product number
- PA5-21685 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PFKL Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: A431, H1299, HeLa, HepG2. Predicted reactivity: Mouse (95%), Rat (95%), Chicken (88%), Bovine (94%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1.23 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Identification of a multienzyme complex for glucose metabolism in living cells.
Kohnhorst CL, Kyoung M, Jeon M, Schmitt DL, Kennedy EL, Ramirez J, Bracey SM, Luu BT, Russell SJ, An S
The Journal of biological chemistry 2017 Jun 2;292(22):9191-9203
The Journal of biological chemistry 2017 Jun 2;292(22):9191-9203
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using PFKL Polyclonal Antibody (Product # PA5-21685). Sample (30 µg of whole cell lysate). A: Hep G2. 7.5% SDS PAGE. PFKL Polyclonal Antibody (Product # PA5-21685) diluted at 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of PFKL in paraformaldehyde-fixed A549 cells using a PFKL polyclonal antibody (Product # PA5-21685) at a 1:200 dilution.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of paraformaldehyde-fixed A549, using PFKL (Product # PA5-21685) antibody at 1:200 dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of PFKL was performed in paraffin-embedded mouse cervix tissue using PFKL Polyclonal Antibody (Product # PA5-21685) at a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded SW480 xenograft, using PFKL (Product # PA5-21685) antibody at 1:500 dilution. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
