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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Other assay [1]
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- Product number
- PA5-30924 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- 4-1BB Ligand Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: A549, HeLa, HepG2, HCT116. Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using 4-1BB Ligand Polyclonal Antibody (Product # PA5-30924). Sample (30 µg of whole cell lysate). Lane A: HCT116. 12% SDS PAGE. 4-1BB Ligand Polyclonal Antibody (Product # PA5-30924) diluted at 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- TNFSF9 antibody [N2C3] immunoprecipitates TNFSF9 protein in IP experiments. IP samples: A549 membrane extract. A. Control with 4 µg of preimmune Rabbit IgG. B. Immunoprecipitation of TNFSF9 protein by 4 µg TNFSF9 antibody [N2C3] (Product # PA5-30924). 15 % SDS-PAGE. The immunoprecipitated TNFSF9 protein was detected by TNFSF9 antibody [N2C3] (Product # PA5-30924) diluted at 1:500.
