AF3277

antibody from Novus Biologicals

Targeting: TRAF2 RNF117, TRAP3

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

AF3277

Provider

Novus Biologicals

Product name

Goat Polyclonal TRAF-2 Antibody

Antibody type

Polyclonal

Description

Antigen Affinity-purified. Detects human TRAF-2 in Western blots. In Western blots, less than 1% cross-reacivity with recombinant human TRAF-1, -3, -4, -5, or -6 is observed.

Reactivity

Human

Host

Goat

Conjugate

Unconjugated

Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

References

Submitted references

Dual role of DR5 in death and survival signaling leads to TRAIL resistance in cancer cells.

Shlyakhtina Y, Pavet V, Gronemeyer H
Cell death & disease 2017 Aug 31;8(8):e3025

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Detection of Human TRAF-2 by Western Blot. Western blot shows lysates of Raji human Burkitt's lymphoma cell line and HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 0.5 µg/mL Goat Anti-Human TRAF-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3277) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). For additional reference, recombinant human TRAF-1, TRAF-2, TRAF-3, TRAF-4, TRAF-5, and TRAF-6 (2 ng/lane) were included. A specific band for TRAF-2 was detected at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.