H00007186-M04

antibody from Abnova Corporation

Targeting: TRAF2 RNF117, TRAP3

Western blot (Supportive data in Antibodypedia)

Proximity ligation assay (Supportive data in Antibodypedia)

Antibody Data

Product number

H00007186-M04

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00007186-M04, RRID:AB_1581464

Product name

TRAF2 monoclonal antibody (M04), clone 4C11

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a full-length recombinant TRAF2.

Antigen sequence

MAAASVTPPGSLELLQPGFSKTLLGTKLEAKYLCS
ACRNVLRRPFQAQCGHRYCSFCLASILSSGPQNCA
ACVHEGIYEEGISILESSSAFPDNAARREVESLPA
VCPSDGCTWKGTLKEYESCHEGRCPLMLTECPACK
GLVRLGEKERHLEHECPERSLSCRHCRAPCCGADV
KAHHEVCPKFPLTCDGCGKKKIPREKFQDHVKTCG
KCRVPCRFHAIGCLETVEGEKQQEHEVQWLREHLA
MLLSSVLEAKPLLGDQSHAGSELLQRCESLEKKTA
TFENIVCVLNREVERVAMTAEACSRQHRLDQDKIE
ALSSKVQQLERSIGLKDLAMADLEQKVLEMEASTY
DGVFIWKISDFARKRQEAVAGRIPAIFSPAFYTSR
YGYKMCLRIYLNGDGTGRGTHLSLFFVVMKGPNDA
LLRWPFNQKVTLMLLDQNNREHVIDAFRPDVTSSS
FQRPVNDMNIASGCPLFCPVSKMEAKNSYVRDDAI
FIKAIVDLTGL


Isotype

IgG

Antibody clone number

4C11

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of TRAF2 expression in transfected 293T cell line by TRAF2 monoclonal antibody (M04), clone 4C11.Lane 1: TRAF2 transfected lysate(55.9 KDa).Lane 2: Non-transfected lysate.

Proximity ligation assay

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Proximity Ligation Analysis of protein-protein interactions between IKBKB and TRAF2. HeLa cells were stained with anti-IKBKB rabbit purified polyclonal 1:1200 and anti-TRAF2 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).

Validation comment

In situ Proximity Ligation Assay (Cell)