H00007186-D01P

antibody from Abnova Corporation

Targeting: TRAF2 RNF117, TRAP3

Western blot (Supportive data in Antibodypedia)

Proximity ligation assay (Supportive data in Antibodypedia)

Antibody Data

Product number

H00007186-D01P

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00007186-D01P, RRID:AB_1716396

Product name

TRAF2 purified MaxPab rabbit polyclonal antibody (D01P)

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against a full-length human TRAF2 protein.

Antigen sequence

MAAASVTPPGSLELLQPGFSKTLLGTKLEAKYLCS
ACRNVLRRPFQAQCGHRYCSFCLASILSSGPQNCA
ACVHEGIYEEGISILESSSAFPDNAARREVESLPA
VCPSDGCTWKGTLKEYESCHEGRCPLMLTECPACK
GLVRLGEKERHLEHECPERSLSCRHCRAPCCGADV
KAHHEVCPKFPLTCDGCGKKKIPREKFQDHVKTCG
KCRVPCRFHAIGCLETVEGEKQQEHEVQWLREHLA
MLLSSVLEAKPLLGDQSHAGSELLQRCESLEKKTA
TFENIVCVLNREVERVAMTAEACSRQHRLDQDKIE
ALSSKVQQLERSIGLKDLAMADLEQKVLEMEASTY
DGVFIWKISDFARKRQEAVAGRIPAIFSPAFYTSR
YGYKMCLRIYLNGDGTGRGTHLSLFFVVMKGPNDA
LLRWPFNQKVTLMLLDQNNREHVIDAFRPDVTSSS
FQRPVNDMNIASGCPLFCPVSKMEAKNSYVRDDAI
FIKAIVDLTGL


Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of TRAF2 expression in transfected 293T cell line (H00007186-T01) by TRAF2 MaxPab polyclonal antibody.Lane 1: TRAF2 transfected lysate(55.90 KDa).Lane 2: Non-transfected lysate.

Proximity ligation assay

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Proximity Ligation Analysis of protein-protein interactions between TRAF2 and ACTG1. HeLa cells were stained with anti-TRAF2 rabbit purified polyclonal 1:1200 and anti-ACTG1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).

Validation comment

In situ Proximity Ligation Assay (Cell)