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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
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- Product number
- PAB16905 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB16905, RRID:AB_10675594
- Product name
- TRAF2 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of TRAF2 .
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references RNAi screen in mouse astrocytes identifies phosphatases that regulate NF-kappaB signaling.
Li S, Wang L, Berman MA, Zhang Y, Dorf ME
Molecular cell 2006 Nov 17;24(4):497-509
Molecular cell 2006 Nov 17;24(4):497-509
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Supportive validation
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- Abnova Corporation (provider)
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- Experimental details
- Western blot using TRAF2 polyclonal antibody (Cat # PAB16905) shows detection of endogenous TRAF2 in whole HeLa cell lysates. Lane 1 shows endogenous TRAF2 detected with antibody at 47 KDa (arrowhead). Lane 2 shows no reactivity when blot is incubated with immunizing peptide. The identity of lower molecular weight band in lane 1 is unknown. Briefly, each lane contains approximately 14 ug of lysate. Membranes were blocked in 3% BSA-TBS 30 min. at room temperature. Primary antibody was used at a 1 : 500 dilution in 3% BSA-TBS and reacted overnight at 4°C. The membrane was washed and reacted with a 1 : 20,000 dilution conjugated Gt-a-Rabbit DyLight 649 for 1 hr at room temperature. Fluorescence image was captured using the VersaDoc®Imaging System developed by Bio-Rad.
