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Western blot
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ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunoprecipitation [1]
- Immunohistochemistry [2]
- Flow cytometry [1]
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- Product number
- MA5-49822 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- TRAF2 Recombinant Rabbit Monoclonal Antibody (9A5)
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Antibody clone number
- 9A5
- Vial size
- 100 µL
- Concentration
- 0.8 mg/mL
- Storage
- -20°C or -80°C if preferred
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation analysis of TRAF2 in HeLa whole cell lysate; Lane 1: Rabbit control IgG. Samples were incubated with TRAF2 monoclonal antibody (Product # MA5-49822).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis with a TRAF2 monoclonal antibody (Product # MA5-49822) using a dilution of 1:100 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis with a TRAF2 monoclonal antibody (Product # MA5-49822) using a dilution of 1:100 and staining in paraffin-embedded human cervical cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry analysis overlay histogram showing Hela cells stained with TRAF2 monoclonal antibody (Product # MA5-49822) (red line) at 1:50. The cells were fixed with 70% Ethyl alcohol (18h) and then incubated in 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1 µg/1x10^6 cells) for 1 h at 4°C.The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 30 min at 4°C. Control antibody (green line) was Rabbit IgG (1 µg/1x10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.
