MA1-41028

antibody from Invitrogen Antibodies

Targeting: TRAF2 RNF117, TRAP3

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Recommended by provider)

Antibody Data

Product number

MA1-41028

Provider

Invitrogen Antibodies

Product name

TRAF2 Monoclonal Antibody (33A1293)

Antibody type

Monoclonal

Antigen

Other

Description

Suggested positive control: Hela whole cell extract, antigen standard for TRAF2 (transient overexpression lysate).

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

33A1293

Vial size

100 µg

Concentration

1.0 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of TRAF2 in HeLa lysate. Samples were incubated in TRAF2 monoclonal antibody (Product # MA1-41028) using a dilution of 2 µg/mL.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Knockdown of TRAF2 was achieved by transfecting HeLa with TRAF2 specific siRNAs (Silencer® select Product # s14379, s14380). Western blot analysis (Fig. a) was performed using whole cell extracts from the TRAF2 knockdown cells (lane 3), non-specific scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blot was probed with TRAF2 Monoclonal Antibody (33A1293) (Product # MA1-41028, 1:1000 dilution) and Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 0.25 µg/mL, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to TRAF2.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot was performed using Anti-TRAF2 Monoclonal Antibody (33A1293), (Product # MA1-41028) and a 61 kDa band corresponding to TRAF2 along with an uncharacterized band (*) at ~35 kDa was observed in the cell lines tested. Whole cell extracts (40 µg lysate) of HeLa (Lane 1), Jurkat (Lane 2), HEK-293 (Lane 3) and Raji (Lane 4) were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0321BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blots were probed with the primary antibody (1 µg/mL) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).