Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Immunocytochemistry [1]
- Flow cytometry [1]
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Validation data
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- Product number
- BAF2255 - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse LDLR Biotinylated Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects mouse LDL R in Western blots. In Western blots, approximately 5% cross-reactivity with recombinant human LDL R is observed.
- Reactivity
- Mouse
- Host
- Goat
- Conjugate
- Biotin
- Antigen sequence
Q6GTJ9
- Isotype
- IgG
- Vial size
- 50 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Annexin A2 is a natural extrahepatic inhibitor of the PCSK9-induced LDL receptor degradation.
Seidah NG, Poirier S, Denis M, Parker R, Miao B, Mapelli C, Prat A, Wassef H, Davignon J, Hajjar KA, Mayer G
PloS one 2012;7(7):e41865
PloS one 2012;7(7):e41865
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- LDL R in RAW 264.7 Mouse Cell Line. LDL R was detected in immersion fixed RAW 264.7 mouse monocyte/macrophage cell line using Goat Anti-Mouse Anti-LDL R Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF2255) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (red; Catalog # NL999) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of LDL R in RAW 264.7 Mouse Cell Line by Flow Cytometry. Serum-deprived RAW 264.7 mouse monocyte/macrophage cell line was stained with Goat Anti-Mouse LDL R Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF2255, filled histogram) or isotype control antibody (Catalog # BAF108, open histogram), followed by Streptavidin-PE (Catalog # F0040).