PA5-46985

antibody from Invitrogen Antibodies

Targeting: LDLR LDLCQ2

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Blocking/Neutralizing (Recommended by provider)

Antibody Data

Product number

PA5-46985

Provider

Invitrogen Antibodies

Product name

LDLR Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

In direct ELISAs, approximately 15% cross-reactivity with recombinant mouse LDL R is observed. Reconstitute at 0.2 mg/mL in sterile PBS.

Reactivity

Human

Host

Goat

Isotype

IgG

Vial size

100 µg

Concentration

0.2 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Knockdown of LDLR was achieved by transfecting PC-3 with LDLR specific siRNAs (Silencer® select Product # s6 and s224007). Western blot analysis (Fig. a) was performed using Whole cell extracts from the LDLR knockdown cells (lane 3), non-targeting scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blot was probed with LDLR Polyclonal Antibody (Product # PA5-46985, 0.1 µg/ml ) and Rabbit anti-Goat IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27014, 1:4000). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to LDLR.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry analysis of LDLR in immersion fixed HepG2 human hepatocellular carcinoma cell line. Samples were incubated in LDLR polyclonal antibody (Product # PA5-46985) using a dilution of 1.7 µg/mL for 3 hours at room temperature followed by NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry analysis of LDLR in immersion fixed HepG2 human hepatocellular carcinoma cell line. Samples were incubated in LDLR polyclonal antibody (Product # PA5-46985) using a dilution of 1.7 µg/mL for 3 hours at room temperature followed by NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of LDLR in formalin fixed paraffin-embedded sections of human liver. Samples were incubated in LDLR polyclonal antibody (Product # PA5-46985) using a dilution of 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown) and counterstained with hematoxylin (blue).