Antibody data
- Product number
- PA5-20752
- Provider
- Invitrogen Antibodies
- Product name
- Anti-LDLR Polyclonal Antibody
- Provider product page
- Invitrogen Antibodies - PA5-20752
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- A suggested positive control is human liver tissue lysate. PA5-20752 can be used with blocking peptide PEP-0866.
- Reactivity
- Human, Mouse, Rat
- Host
- Chicken/Avian
- Isotype
- IgY
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C
Supportive validation
- Submitted by
-
Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of human liver tissue lysate using a LDL-R polyclonal antibody (Product # PA5-20752) at (A) 1 and (B) 2 µg/mL.
Supportive validation
- Submitted by
-
Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis was performed on whole cell extracts (30 µg lysate) of WJ MSC (Lane 1), WJ MSC treated with BMP-2 (100ng/ml for 21 days) (Lane 2), HeLa (Lane 3), Hep G2 (Lane 4), PC-3 (Lane 5) and LNCaP (Lane 6). The blot was probed with Anti-LDLR Antibody (35H2L48) (Product # PA5-20752, 1 µg/ml) and detected by chemiluminescence using Goat anti-Chicken IgY (H+L), HRP, Polyclonal, Secondary Antibody, Invitrogen™, (Product # A16054, 0.25 µg/ml, 1:4000 dilution). A 80 kDa band corresponding to LDLR was observed in the cell line tested and decreased upon BMP treatment in WJ MSC cell line. A non-specific 40 kDa band was observed in the cell lines tested.
Supportive validation
- Submitted by
-
Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of human liver tissue using a LDL-R polyclonal antibody (Product # PA5-20752) at a 20 µg/mL dilution.
Supportive validation
- Submitted by
-
Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of human liver tissue using a LDL-R polyclonal antibody (Product # PA5-20752) at a 25 µg/mL dilution.
Supportive validation
- Submitted by
-
Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of human liver tissue using a LDL-R polyclonal antibody (Product # PA5-20752) at a 20 µg/mL dilution.
Supportive data in Antibodypedia
