PA5-20752

antibody from Invitrogen Antibodies

Targeting: LDLR LDLCQ2

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-20752

Provider

Invitrogen Antibodies

Product name

LDLR Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

A suggested positive control is human liver tissue lysate.

Concentration

1 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of human liver tissue lysate using a LDL-R polyclonal antibody (Product # PA5-20752) at (A) 1 and (B) 2 µg/mL.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of LDL-R in human liver tissue lysate with LDLR Polyclonal Antibody (Product # PA5-20752) at (A) 1 and (B) 2 µg/mL.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis was performed on whole cell extracts (30 µg lysate) of WJ MSC (Lane 1), WJ MSC treated with BMP-2 (100ng/ml for 21 days) (Lane 2), HeLa (Lane 3), Hep G2 (Lane 4), PC-3 (Lane 5) and LNCaP (Lane 6). The blot was probed with Anti-LDLR Antibody (35H2L48) (Product # PA5-20752, 1 µg/ml) and detected by chemiluminescence using Goat anti-Chicken IgY (H+L), HRP, Polyclonal, Secondary Antibody, Invitrogen™, (Product # A16054, 0.25 µg/ml, 1:4000 dilution). A 80 kDa band corresponding to LDLR was observed in the cell line tested and decreased upon BMP treatment in WJ MSC cell line. A non-specific 40 kDa band was observed in the cell lines tested.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of human liver tissue using a LDL-R polyclonal antibody (Product # PA5-20752) at a 20 µg/mL dilution.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence of LDLR in human liver tissue with LDLR Polyclonal Antibody (Product # PA5-20752) at 20 µg/mL.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of LDL-R in human liver tissue with LDLR Polyclonal Antibody (Product # PA5-20752) at 2.5 µg/mL.