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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- AP32532PU-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Product name
- anti PRKCH pThr655
- Antibody type
- Polyclonal
- Antigen
- Peptide corresponding to amino acid region encompassing the Human, Mouse, and Rat phospho-PKCη (Thr655).
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
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Supportive validation
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- Acris Antibodies GmbH (provider)
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- Experimental details
- Lysates from PMA treated Jurkat cells were resolved by electrophoresis, transferred to nitrocellulose, and probed with anti-phospho-PKCη (Ser655) for 2 hours at room temperature. Lane 2 was pre-incubated with the blocking peptide immunogen corresponding to phospho-PKCη (Ser655). Lane 3 was pre-incubated with lambda phosphatase prior to primary antibody incubation. Proteins were visualized via HRP and chemiluminescent detection. Arrow indicates phospho-PKCη.
