PA5-92379

antibody from Invitrogen Antibodies

Targeting: ABAT GABA-T, GABAT

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-92379

Provider

Invitrogen Antibodies

Product name

ABAT Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Immunogen sequence: SQAAAKVDVE FDYDGPLMKT EVPGPRSQEL MKQLNIIQNA EAVHFFCNYE ESRGNYLVDV DGNRMLDLYS QISSVPIGYS HPALLKLIQQ PQNASMFVNR PALGILPPEN FVEKLRQSLL SVAPKGMSQL ITMACGSCSN ENALKTIFMW YRSKERGQRG FSQEELETCM INQAPGCPDY SILSFMGAFH GRTMGCLATT HSKAIHKIDI PSFDWPIAPF PRLKYPLEEF VKENQQEEAR CLEEVEDLIV KYRKKKKTVA GIIVEPIQSE GG; Positive Samples: SW480, A375, Mouse liver, Mouse kidney, Mouse brain; Cellular Location: Mitochondrion matrix

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1.04 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of extracts of various cell lines, using ABAT Polyclonal antibody (Product # PA5-92379) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Exposure time: 10s.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of ABAT in extracts of various cell lines using ABAT Polyclonal Antibody (Product # PA5-92379) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of ABAT in extracts of HepG2 cells using ABAT Polyclonal Antibody (Product # PA5-92379) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of ABAT was performed in A549 cells using ABAT Polyclonal Antibody (Product # PA5-92379).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of ABAT in paraffin-embedded rat kidney using ABAT Polyclonal Antibody (Product # PA5-92379) at a dilution of 1:100.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of ABAT in paraffin-embedded human liver damage using ABAT Polyclonal Antibody (Product # PA5-92379) at a dilution of 1:200.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of ABAT in paraffin-embedded human gastric cancer using ABAT Polyclonal Antibody (Product # PA5-92379) at a dilution of 1:200.