Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PA5-27864 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- MSH3 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: A549, H1299, HCT116. Predicted reactivity: Rat (81%), Bovine (83%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Evolving neoantigen profiles in colorectal cancers with DNA repair defects.
Rospo G, Lorenzato A, Amirouchene-Angelozzi N, Magrì A, Cancelliere C, Corti G, Negrino C, Amodio V, Montone M, Bartolini A, Barault L, Novara L, Isella C, Medico E, Bertotti A, Trusolino L, Germano G, Di Nicolantonio F, Bardelli A
Genome medicine 2019 Jun 28;11(1):42
Genome medicine 2019 Jun 28;11(1):42
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of MSH3 using A) 30 µg A549 whole cell lysate (B) 30 µg H1299 whole cell lysate and C) 30 µg HCT116 whole cell lysate. Samples were loaded onto a 5% SDS-PAGE gel and probed with a MSH3 polyclonal antibody (Product # PA5-27864) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- MSH3 antibody detects MSH3 protein by Western blot analysis. A. 30 µg A549 whole cell lysate/extract. B. 30 µg H1299 whole cell lysate/extract. C. 30 µg HCT116 whole cell lysate/extract.5 % SDS-PAGE. MSH3 antibody MSH3 Polyclonal Antibody (Product # PA5-27864) dilution: 1:1,000.
