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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [1]
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Validation data
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- Product number
- 100-401-A22 - Provider product page
- Provider
- Rockland Immunochemicals, Inc.
- Proper citation
- Rockland Cat#100-401-A22, RRID:AB_2194566
- Product name
- Anti-Selenophosphate Synthetase 1 (SPS1) (RABBIT) Antibody - 100-401-A22
- Antibody type
- Polyclonal
- Vial size
- 100 µl
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Supportive validation
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- Rockland Immunochemicals, Inc. (provider)
- Main image
- Experimental details
- Western blot using Rockland's anti-SPS1 antibody shows detection of endogenous SPS1 in NIH3T3 cell. No signal is seen in lysates from cells after pre-treatment with SPS1 siRNA. Endogenous SPS1 can be detected in mouse kidney and liver tissue lysates. Negligible cross-reactivity is seen against recombinant SPS2. The primary antibody was used at a 1:1000 dilution. Personal Communication, D. Hatfield, NCI, Bethesda, MD.
- Validation comment
- Western Blot
