Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunocytochemistry [1]
- Immunohistochemistry [2]
- Flow cytometry [2]
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Validation data
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- Product number
- MA5-29000 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ADAM15 Recombinant Rabbit Monoclonal Antibody (007)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%). Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire. This antibody has specificity for Human ADAM15/MDC15.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Antibody clone number
- 7
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence staining of Human ADAM15 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 1% Triton X-100 in PBS, blocked with 10% serum, and incubated with ADAM15 Recombinant Rabbit Monoclonal Antibody (7) (Product # MA5-29000, 1:60) at 37°C 1 hour. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to cytoplasm.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of formalin fixed, paraffin-embedded human heart showing blood vessel staining (red arrow) using ADAM15 Recombinant Rabbit Monoclonal Antibody (7) (Product # MA5-29000, 1:200).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of formalin fixed, paraffin-embedded human lymph node showing membrane staining of endothelial cells (red arrow) using ADAM15 Recombinant Rabbit Monoclonal Antibody (7) (Product # MA5-29000, 1:2,000).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Profile of anti-ADAM15 reactivity on MCF-7 cells analyzed by flow cytometry. Cells were stained with an ADAM15 Monoclonal Antibody (Product # MA5-29000)
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Profile of ADAM15 on MCF-7 cells analyzed by flow cytometry using ADAM15 Recombinant Rabbit Monoclonal Antibody (7) (Product # MA5-29000).