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Flow cytometryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Flow cytometry [1]
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- Product number
- IC935P - Provider product page
- Provider
- R&D Systems
- Product name
- Human ADAM15 Ectodomain PE-conjugated Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects the ectodomain of recombinant human (rh) ADAM15 in direct ELISAs and Western blots. In direct ELISAs, shows 100% cross-reactivity with recombinant mouse (rm) ADAM15 but does not cross-react with rhADAM8, 9, 17, 28, rmADAM9, or 10.
- Reactivity
- Human
- Host
- Mouse
- Antigen sequence
Q13444- Isotype
- IgG
- Antibody clone number
- 23G9
- Vial size
- 100 Tests
- Storage
- Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied.
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- Detection of ADAM15 in MCF-7 Human Cell Line by Flow Cytometry. MCF-7 human breast cancer cell line was stained with Mouse Anti-Human ADAM15 Ectodomain PE-conjugated Monoclonal Antibody (Catalog # IC935P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
