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LearnPAB10023
antibody from Abnova Corporation
Targeting: DCLRE1C
A-SCID, ARTEMIS, FLJ11360, SCIDA, SNM1C
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- PAB10023 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB10023, RRID:AB_1671809
- Product name
- DCLRE1C polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Goat polyclonal antibody raised against synthetic peptide of DCLRE1C.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Functional and biochemical dissection of the structure-specific nuclease ARTEMIS.
Radiosensitive SCID patients with Artemis gene mutations show a complete B-cell differentiation arrest at the pre-B-cell receptor checkpoint in bone marrow.
Novel Artemis gene mutations of radiosensitive severe combined immunodeficiency in Japanese families.
Pannicke U, Ma Y, Hopfner KP, Niewolik D, Lieber MR, Schwarz K
The EMBO journal 2004 May 5;23(9):1987-97
The EMBO journal 2004 May 5;23(9):1987-97
Radiosensitive SCID patients with Artemis gene mutations show a complete B-cell differentiation arrest at the pre-B-cell receptor checkpoint in bone marrow.
Noordzij JG, Verkaik NS, van der Burg M, van Veelen LR, de Bruin-Versteeg S, Wiegant W, Vossen JM, Weemaes CM, de Groot R, Zdzienicka MZ, van Gent DC, van Dongen JJ
Blood 2003 Feb 15;101(4):1446-52
Blood 2003 Feb 15;101(4):1446-52
Novel Artemis gene mutations of radiosensitive severe combined immunodeficiency in Japanese families.
Kobayashi N, Agematsu K, Sugita K, Sako M, Nonoyama S, Yachie A, Kumaki S, Tsuchiya S, Ochs HD, Sugita K, Fukushima Y, Komiyama A
Human genetics 2003 Apr;112(4):348-52
Human genetics 2003 Apr;112(4):348-52
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunoblotting of DCLRE1C polyclonal antibody (Cat # PAB10023) was used at a 1 : 500 dilution to detect DCLRE1C is various cell lysates by Western blot.CJ is an DCLRE1C deficient cell lineand so no band is visible.This antibody detects a band of approximately 90 KDa (predicted molecular weight: 78 KDa).
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunohistochemistry of DCLRE1C polyclonal antibody (Cat # PAB10023) was used at a 1 : 1000 dilution to detect DCLRE1C by immunohistochemistry in human spleen.Positive staining of T cells and B lymphocytes is observed in thymus, lymph nodes and spleen.Tissue was formalin-fixed and paraffin embedded.Personal Communication, Alan Yen, Life Span Biosciences, Seattle, WA.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
