Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PA5-50479 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PYGL Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- The antibody detects endogenous levels of total PYGL protein.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 2 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of PYGL was performed by loading (from left to right): 231 and PC3 cell lysates (40µg) on to a 6% SDS-PAGE gel. Proteins were transferred to a membrane and the membrane was probed with a PYGL antibody (Product # PA5-50479) at a 1/400 dilution for 20 seconds, followed by a secondary antibody at 1/8000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of PYGL was performed by loading whole-cell lysates of four different human cell lines. PYGL was detected at approximately 95 kDa by using a PYGL polyclonal Antibody (Product # PA5-50479) at a concentration of 2.0 µg/mL in Blocking Buffer overnight at 4°C on a rocking platform, followed by a 1-hour room-temperature incubation of a donkey anti-rabbit IgG antibody at a dilution of 1:5,000. Data courtesy of Thermo Scientific KOL Program.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of PYGL in paraffin embedded Human thyroid cancer tissue using PYGL Polyclonal Antibody (Product # PA5-50479) at a 1:35 dilution.