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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- Immunocytochemistry [2]
- Immunohistochemistry [7]
- Other assay [6]
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- Product number
- PA5-51492 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PYGL Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: MRIDDVAALD KKGYEAKEYY EALPELKLVI DQIDNGFFSP KQPDLFKDII NMLFYHDRFK VFADYEAYVK CQDKVSQLYM NPKAWNTMVL KNIAASGKFS SDRTIKEYAQ NIWNVEPSD
- Concentration
- 0.1 mg/mL
Submitted references Breast cancers utilize hypoxic glycogen stores via PYGB, the brain isoform of glycogen phosphorylase, to promote metastatic phenotypes.
Altemus MA, Goo LE, Little AC, Yates JA, Cheriyan HG, Wu ZF, Merajver SD
PloS one 2019;14(9):e0220973
PloS one 2019;14(9):e0220973
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of PYGL in human cell lines PC-3 and SK-MEL-30 using a PYGL Polyclonal Antibody (Product # PA5-51492). Corresponding PYGL RNA-seq data are presented for the same cell lines. Loading control: Anti-HSP90B1.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of PYGL in human cell line U-2 OS shows positivity in plasma membrane & cytoplasm. Samples were probed using a PYGL Polyclonal Antibody (Product # PA5-51492).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of PYGL in human cell line U-251 MG using a PYGL Polyclonal Antibody (Product # PA5-51492) shows localization to nucleoplasm, plasma membrane and cytosol.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of PYGL in human testis using PYGL Polyclonal Antibody (Product # PA5-51492).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of PYGL in human liver tissue shows strong cytoplasmic positivity in hepatocytes. Samples were probed using a PYGL Polyclonal Antibody (Product # PA5-51492).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of PYGL in human liver and pancreas tissues using PYGL Polyclonal Antibody (Product # PA5-51492). Corresponding PYGL RNA-seq data are presented for the same tissues.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of PYGL in human bone marrow, cerebral cortex, liver and testis using PYGL Polyclonal Antibody (Product # PA5-51492) (A) shows similar protein distribution across tissues to an independent PYGL Polyclonal Antibody (B).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of PYGL in human pancreas using PYGL Polyclonal Antibody (Product # PA5-51492) shows low expression as expected.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of PYGL in human bone marrow using PYGL Polyclonal Antibody (Product # PA5-51492).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of PYGL in human cerebral cortex using PYGL Polyclonal Antibody (Product # PA5-51492).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fig 3 Glycogen phosphorylase brain isoform knockdown inhibits glycogen utilization in breast cancer cells. (A) Western blot of liver and brain glycogen phosphorylase (PYGL and PYGB) in wild-type, either empty vector, shPYGL and shPYGB transduced MDA-MB-231, MCF-7, and MCF-10A cells. Actin is shown as a loading control(B-D) Glycogen levels normalized to protein in lysate after 24h in hypoxia (Hypoxic Control) or 24h in hypoxia with subsequent 24h in normoxia (Normoxic Exposure) for wild-type, empty vector, shPYGL, and shPYGB in (B) MDA-MB-231, (C) MCF-7, and (D) MCF-10A cells, respectively. Error bars represent SEM of n = 3. *p
